anti active caspase 3  (R&D Systems)

Journal: Nature Genetics

Article Title: Single-cell spatial transcriptomic analysis of human skin anatomy

doi: 10.1038/s41588-026-02552-8

Figure Lengend Snippet: a , Schematic of human skin explant workflow (left) with representative images of hematoxylin and eosin staining (right) of freshly collected skin or explants cultured for 48 h. Scale bar, 100 mm. b , Representative immunofluorescence staining of activated Caspase-3 (aCASPASE-3) (red) and DAPI (blue) in freshly collected skin or explants cultured for 48 h showing minimal expression in both conditions. Dotted white line indicates basement membrane. Similar results for donor 1 are not shown. c , Schematic of human skin explant workflow (left), representative images of RNA FISH of CCL19 (red) and PDGFRA (green) on sections from freshly collected skin or explants cultured for 48 h treated with vehicle control or recombinant TNF (10 ng ml −1 ) (center), and quantification of number of PDGFRA + / CCL19 + cells per high-powered field (HPF) (right). Dotted white line indicates basement membrane. Data are mean ± s.e.m. P values shown calculated with two-tailed t -test. d , Additional representative RNA FISH images of superficial perivascular areas of freshly collected skin or human skin explants treated with vehicle control or recombinant TNF (10 ng ml −1 ). e , qPCR of CCL19 in human skin explants cultured in presence of absence of recombinant TNF (10 ng ml −1 ). Data are mean ± s.e.m. *, P < 0.05 by paired one-tailed t -test.

Article Snippet: Blocking was performed with 5.0% normal donkey serum (Jackson ImmunoResearch), 1% BSA (Sigma-Aldrich) and 0.3% Triton X in PBS for 1 h. Sections were incubated overnight at 4 °C with anti-activated Caspase-3 (R&D systems, AF835; 1:100), followed by donkey antirabbit Alexa Fluor 647 secondary antibody (Jackson ImmunoResearch; 1:400) for 1 h at room temperature.

Techniques: Staining, Cell Culture, Immunofluorescence, Expressing, Membrane, Control, Recombinant, Two Tailed Test, One-tailed Test

Journal: Stem Cell Reports

Article Title: Histone methyltransferase Setd8 preserves chromatin accessibility to safeguard retinal progenitor cell identity during development

doi: 10.1016/j.stemcr.2025.102789

Figure Lengend Snippet: Setd8 deficiency reduces RPC proliferation and promotes apoptosis (A and D) Representative retinal section images of YFP (green) and EdU (magenta) staining at P0 (A) and P2 (D) from Ctrl (left) and Setd8 cKO (right) mice. Scale bars, 50 μm. (B and E) Quantification of the number of YFP+ (left graph) or EdU+ (right graph) cells at P0 (B) and P2 (E) ( n = 15 from 5 retinas). ∗∗ p < 0.01. (C and F) Proportion of YFP+ EdU+ (double-positive) cells among YFP+ cells at P0 (C) and P2 (F) ( n = 15 from 5 retinas). ∗∗ p < 0.01. (G) Representative retinal section images of γH2AX (magenta) staining at P0 (left) and P2 (right) from Ctrl (upper) and Setd8 cKO (lower) mice. Scale bars, 50 μm. (H) Quantification of the number of γH2AX + cells at P0 and P2 ( n = 15 from 5 retinas). (I) Representative retinal section images of YFP (green) and active caspase-3 (magenta) staining at P0 (left) and P2 (right) from Ctrl (upper) and Setd8 cKO (lower) mice. Scale bars, 50 μm. (J) Quantification of active caspase-3+ cells at P0 and P2 ( n = 15 from 5 retinas).

Article Snippet: The following primary antibodies were used: chicken anti-Nestin (1:500, NES, Aves Labs); goat anti-Sox2 (1:500, AF2018, R&D Systems); rabbit anti-active caspase 3 (1:500, AF-835, R&D Systems); chick anti-GFP (1:500, GFP-1010, Aves Labs); sheep anti-Chx10 (1:100, X1180P, Exalpha); rabbit anti-H4K20me1 (1:500, ab9051, Abcam); rabbit anti-S-opsin (1:500, AB5407, Millipore); rabbit anti-M-opsin (1:200, AB5405, Millipore); mouse anti-Brn3a (1:100, MAB1585, Millipore); mouse anti-Calbindin (1:500, CB300, Swant); goat anti-Otx2 (1:100, BAF1979, R&D Systems); mouse anti-Glutamine synthetase (1:500, 610517, BD Biosciences); rabbit anti-Pax6 (1:500, 901301, BioLegend); rabbit anti-γH2AX (1:500, ab2893, Abcam); and rat anti-BrdU (1:500, ab-6326, Abcam).

Techniques: Staining